Construction of a large signature-tagged mini-Tn5 transposon library and its application to mutagenesis of Sinorhizobium meliloti.

@article{Pobigaylo2006ConstructionOA,
  title={Construction of a large signature-tagged mini-Tn5 transposon library and its application to mutagenesis of Sinorhizobium meliloti.},
  author={Nataliya Pobigaylo and Danijel Wetter and Silke Szymczak and Ulf D. Schiller and Stefan Kurtz and Folker Meyer and Tim W. Nattkemper and Anke Becker},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 6},
  pages={4329-37}
}
Sinorhizobium meliloti genome sequence determination has provided the basis for different approaches of functional genomics for this symbiotic nitrogen-fixing alpha-proteobacterium. One of these approaches is gene disruption with subsequent analysis of mutant phenotypes. This method is efficient for single genes; however, it is laborious and time-consuming if it is used on a large scale. Here, we used a signature-tagged transposon mutagenesis method that allowed analysis of the survival and… CONTINUE READING