Construction of a host-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii

@inproceedings{Ushio1988ConstructionOA,
  title={Construction of a host-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii},
  author={Kohei Ushio and Hiroki Tatsumi and Hiroyuki Araki and Akio Toh-E and Yasuji Oshima},
  year={1988}
}
Abstract A host-vector system for Zygosaccharomyces rouxii was developed. Chimeric plasmids useful as the Escherichia coli-Z. rouxii shuttle vector were constructed with a DNA fragment of pBR322, a fragment of pSR1 plasmid of Z. rouxii or the ARS1 sequence of Saccharomyces cerevisiae, and a fragment of the LEU2 gene of S. cerevisiae or a DNA fragment bearing Tn601 which confers G-418 resistance as a selective marker of the plasmid. For the hosts, wild-type strains of Z. rouxii were modified to… CONTINUE READING