Construction of a host-vector system in Candida maltosa by using an ARS site isolated from its genome.

@article{Takagi1986ConstructionOA,
  title={Construction of a host-vector system in Candida maltosa by using an ARS site isolated from its genome.},
  author={Masamichi Takagi and Shinya Kawai and Michelle C Y Chang and Izumi Shibuya and Ken-ichi Yano},
  journal={Journal of bacteriology},
  year={1986},
  volume={167 2},
  pages={
          551-5
        }
}
To construct a host-vector system in an n-alkane-assimilating yeast, Candida maltosa, the isolation of an ARS site from its genome which replicates autonomously in C. maltosa was attempted. Leu- mutants of C. maltosa were transformed with a gene library prepared by using YEp13 (LEU2+) as a vector, and Leu+ transformants were obtained at a high frequency. A plasmid named pCS1 was isolated from the recipient cells. pCS1 contained a 6.3-kilobase (kb) fragment of the C. maltosa genome, and a 3.8-kb… CONTINUE READING
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