Construction of a herpes simplex virus/varicella-zoster virus (HSV/VZV) thymidine kinase recombinant with the pathogenic potential of HSV and a drug sensitivity profile resembling that of VZV.

@article{Bevilacqua1995ConstructionOA,
  title={Construction of a herpes simplex virus/varicella-zoster virus (HSV/VZV) thymidine kinase recombinant with the pathogenic potential of HSV and a drug sensitivity profile resembling that of VZV.},
  author={F Bevilacqua and Nick J. Davis-Poynter and J Worrallo and D. A. Gower and Peter Collins and Graham Darby},
  journal={The Journal of general virology},
  year={1995},
  volume={76 ( Pt 8)},
  pages={
          1927-35
        }
}
A recombinant of herpes simplex virus (HSV) was constructed in which the HSV thymidine kinase (TK) gene was deleted and the varicella-zoster virus (VZV) TK gene was introduced into the US5 region under the control of the human cytomegalovirus IE promoter. Infection with the recombinant (R18) led to the induction of TK although the kinetics of synthesis resembled those of a 'late' gene product. The recombinant was virulent in the zosteriform mouse model with the pattern of pathogenesis similar… 
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The crystal structure of Varicella zoster virus (VZV, human herpes virus 3) thymidine kinase complexed with (E)-5-(2-bromovinyl)-2′-deoxyuridine 5′-monophosphate and ADP is determined and should be of use in the design of novel antiherpes and antitumor drugs.
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Three recombinant cytomegaloviruses carrying the enhanced yellow fluorescent protein (EYFP) fused with the viral proteins IE-2, ppUL32, and ppUL83 behaved all like wild type, even at low multiplicity of infection (MOI).
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The results show that the DPV Us5 gene is a late gene and that the Us5 protein is a component of the virion, is localized in the cytoplasm, and can inhibit apoptosis induced by H2O2 in DEF cells.
Animal models in virology.
  • O. Weber
  • Biology
    Contributions to microbiology
  • 2001

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