Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius L.).

@article{Boersma2005ConstructionOA,
  title={Construction of a genetic linkage map using MFLP and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius L.).},
  author={Jeffrey G. Boersma and Margaret Pallotta and Chengdao Li and Bevan J. Buirchell and Krishnapillai Sivasithamparam and Huaan Yang},
  journal={Cellular & molecular biology letters},
  year={2005},
  volume={10 2},
  pages={331-44}
}
A mapping population of F(8)derived recombinant inbred lines (RILs) was established from a cross between a domesticated breeding line 83A:476 and a wild type P27255 in narrow-leaf lupin (Lupinus angustifolius L.). The parents together with the 89 RILs were subjected to DNA fingerprinting using microsatellite-anchored fragment length polymorphism (MFLP) to rapidly generate DNA markers to construct a linkage map. Five hundred and twenty two unique markers of which 21% were co-dominant, were… CONTINUE READING
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