Construction of a fully synthetic human scFv antibody library with CDR3 regions randomized by a split-mix-split method and its application.

Abstract

The randomization scheme of hypervariable region takes crucial role in construction of a synthetic antibody library. The codon bias and inevitable 'stop' codon of conventional 'NNK' and 'NNS' codons limit their applications. Here we report a split-mix-split DNA synthesis method that can control over the amino acid composition and distribution of randomized… (More)
DOI: 10.1093/jb/mvn103

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