Construction of a form of the MoFe protein of nitrogenase that accepts electrons from the Fe protein but does not reduce substrate.

Abstract

The direction of electron flow through nitrogenase is generally believed to be from the Fe protein to the P-clusters to the FeMo cofactor and then to substrate. In order to examine oxidation states of the P-clusters that might be involved in this pathway, we have constructed a form of the MoFe protein that contains a species called the MoFe cluster (Gavini… (More)

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