Construction of a cDNA library from polyadenylated RNA of Bacillus subtilis and the determination of some 3'-terminal sequences.

We had found previously that polyadenylated RNA constitutes a surprisingly large fraction of mRNA in both Escherichia coli and Bacillus subtilis [Gopalakrishna et al., Nucl. Acids Res. 9 (1981) 3545-3554; Biochem. 21 (1982) 2724-2729]. We have also shown [Gopalakrishna and Sarkar, J. Biol. Chem. 257 (1982) 2747-2750] that polyadenylated RNA from B. subtilis… (More)