Construction of a 1.2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata (L.) Raf.

@article{Yang2001ConstructionOA,
  title={Construction of a 1.2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata (L.) Raf.},
  author={Zunfu Yang and Xiang Rong Ye and Sang-Haeng Choi and Joe Molina and Francis Moonan and Rod A. Wing and Mikeal Roose and Theodore Erik Mirkov},
  journal={Genome},
  year={2001},
  volume={44 3},
  pages={
          382-93
        }
}
The citrus tristeza virus resistance gene (Ctv) is a single dominant gene in Poncirus trifoliata, a sexually compatible relative of citrus. To clone this gene, a bacterial artificial chromosome (BAC) library has been constructed from an individual plant that was homozygous for Ctv. This library contains 45,696 clones with an average insert size of 80 kb, corresponding to 9.6 genome equivalents. Screening of the BAC library with five chloroplast DNA probes indicated that 0.58% of the BAC clones… CONTINUE READING

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