Construction of Recombinant Marek′s Disease Virus Expressing Avian Influenza Virus HA Gene

@inproceedings{Hongjun2007ConstructionOR,
  title={Construction of Recombinant Marek′s Disease Virus Expressing Avian Influenza Virus HA Gene},
  author={Wang Hong-jun},
  year={2007}
}
In this study,a 1.7 kb DNA fragment encoding the HA gene of Avian influenza virus H9N2 was obtained by RT-PCR and the sequence was cloned into the vector of pcDNA;Then the expression cassette including the HA gene of AIV and LacZ gene of Ecoli controlled by CMV promoter,were inserted into US2 gene of MDV to give a transfer vector.The complex of pUS2 HA and DOTAP was transfected into CEF infected MDV.The recombinant MDV were selected and purified by blue plague.The complete HA gene inserted into… CONTINUE READING

Similar Papers

Loading similar papers…