Corpus ID: 87382024

Construction of Npu DnaE intein and its highly efficient protein trans-splicing activity in 293T cells

@article{Jia2013ConstructionON,
  title={Construction of Npu DnaE intein and its highly efficient protein trans-splicing activity in 293T cells},
  author={Z. Jia},
  journal={Shandong Medical Journal},
  year={2013}
}
  • Z. Jia
  • Published 2013
  • Biology
  • Shandong Medical Journal
  • Objective To construct Npu DnaE intein and to prove that Npu DnaE have highly efficient protein transsplicing activity in 293T cells. Methods We inserted the prepared fusion gene Vn-NDn-myc and NDc-Vc into pCDHCMV-MCS-EF1-Puro vector to construct the plasmid,then the plasmid was transfected into 293T cells,after that we observed the target protein to find the product of Venus with fluorescence microscope. We collected the protein after 48 hours and further proved the Venus protein by using… CONTINUE READING