Construction of N-glycan microarrays by using modular synthesis and on-chip nanoscale enzymatic glycosylation.

@article{Serna2010ConstructionON,
  title={Construction of N-glycan microarrays by using modular synthesis and on-chip nanoscale enzymatic glycosylation.},
  author={Sonia Serna and Juan Etxebarria and Nerea Hernanz Ruiz and Manuel Mart{\'i}n-Lomas and Niels-Christian Reichardt},
  journal={Chemistry},
  year={2010},
  volume={16 44},
  pages={
          13163-75
        }
}
An effective chemoenzymatic strategy is reported that has allowed the construction, for the first time, of a focused microarray of synthetic N-glycans. Based on modular approaches, a variety of N-glycan core structures have been chemically synthesized and covalently immobilized on a glass surface. The printed structures were then enzymatically diversified by the action of three different glycosyltransferases in nanodroplets placed on top of individual spots of the microarray by a printing robot… CONTINUE READING

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