Construction of Modified pTRG Vector as pTRGQQ used in Bacterial Two Hybrid Assay for Protein-Protein Interaction Studies of Dengue Virus Type-2 Protease with Peptide Libraries

Abstract

In molecular biology for cloning experiments, plasmid, a circular molecule is used as vector (carrier) to insert a desired gene or DNA fragment by ligating into it after digestion of both with the compatible restriction enzymes. Then by transforming with a host (generally E. coli) strain of desired traits the screening for appropriate clone is done. A… (More)

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