Construction of Influenza Virus siRNA Expression Vectors and Their Inhibitory Effects on Multiplication of Influenza Virus

@inproceedings{Yaochen2005ConstructionOI,
  title={Construction of Influenza Virus siRNA Expression Vectors and Their Inhibitory Effects on Multiplication of Influenza Virus},
  author={Li Yao-chen and Kong Ling-hong and Chen Bi-zhen and Li Kang-sheng},
  year={2005}
}
Abstract Three plasmid constructs were prepared that express small interfering RNAs (siRNAs) targeted to sequences encoding the ribonucleoprotein member, nucleoprotein (NP) and/or PA, of influenza virus genome. The antiviral properties of siRNAs against the H5N1 strain of influenza virus were studied by evaluating their capacity to silence expression of target genes as well as their effect on influenza virus–induced apoptosis in Madin–Darby canine kidney cells, chicken embryo fibroblast cells… Expand
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References

SHOWING 1-10 OF 32 REFERENCES
RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription
TLDR
It is shown that short interfering RNAs (siRNAs) specific for conserved regions of the viral genome can potently inhibit influenza virus production in both cell lines and embryonated chicken eggs, suggesting that viral mRNA is the target of RNA interference. Expand
Inhibition of influenza virus production in virus-infected mice by RNA interference.
TLDR
It is shown that short interfering RNAs (siRNAs) specific for conserved regions of influenza virus genes can prevent and treat influenza virus infection in mice and development of delivery systems that may be compatible with human use demonstrates the potential utility of siRNAs for prophylaxis and therapy of influenzairus infections in humans. Expand
Protection against lethal influenza virus challenge by RNA interference in vivo.
TLDR
It is demonstrated that treatment with small interfering RNAs (siRNAs) specific for highly conserved regions of the nucleoprotein or acidic polymerase inhibits influenza A virus replication in vivo and that RNA interference is promising for control of influenza virus infection. Expand
Inhibition of virus replication by RNA interference.
TLDR
This review will summarize the current data on RNAi-mediated inhibition of virus replication and discuss the possibilities for the development ofRNAi-based antiviral therapeutics. Expand
The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication.
TLDR
The purpose of this review is to illustrate using the influenza virus NP as a well-studied example that the molecule is much more than a structural RNA-binding protein, but also functions as a key adapter molecule between virus and host cell processes. Expand
In vitro and in vivo anti-influenza A virus activity of antisense oligonucleotides.
TLDR
It is demonstrated that antisense phosphorothioate oligonucleotides (S-ODNs) inhibit influenza virus A replication in MDCK cells and significantly prolonged the mean survival time in day (MDS) and increased the survival rates with does dependent manner. Expand
Antisense therapy of influenza.
  • T. Abe, T. Mizuta, +6 authors H. Takaku
  • Biology, Medicine
  • European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
  • 2001
TLDR
It is demonstrated the first successful in vivo antiviral activity of antisense administered i.v. in experimental respiratory tract infections induced with influenza virus A. Expand
PA Subunit from Influenza Virus Polymerase Complex Interacts with a Cellular Protein with Homology to a Family of Transcriptional Activators
ABSTRACT The PA subunit of the influenza virus polymerase complex is a phosphoprotein that induces proteolytic degradation of coexpressed proteins. Point mutants with reduced proteolysis inductionExpand
Inhibition of influenza virus replication in cultured cells by RNA‐cleaving DNA enzyme
TLDR
Although the RNA‐cleaving activity was not optimal under physiological conditions, DNA enzymes inhibited viral replication in cultured cells more effectively than antisense phosphothioate oligonucleotides, indicating that DNA enzymes could be useful for the control of viral infection. Expand
Induction of programmed cell death (apoptosis) by influenza virus infection in tissue culture cells.
TLDR
It is suggested that influenza virus infection causes apoptotic death of cultured cells, and their fate might be determined at an early stage of the infection by induction of an apoptotic gene. Expand
...
1
2
3
4
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