Construction of Hfr-like donors of the obligate methanol-oxidizing bacterium Methylobacillus flagellatum KT.

@article{Serebrijski1989ConstructionOH,
  title={Construction of Hfr-like donors of the obligate methanol-oxidizing bacterium Methylobacillus flagellatum KT.},
  author={I G Serebrijski and S M Kazakova and Yuri D. Tsygankov},
  journal={FEMS microbiology letters},
  year={1989},
  volume={50 1-2},
  pages={203-6}
}
The transposon-loaded plasmid pAS8-121, incapable of autonomous replication in Gram-negative bacteria of non-enteric group, was transferred to Methylobacillus flagellatum KT wild type strain MFK1. The transconjugants arose at a frequency of 10(-7) per donor cell. The majority of the transconjugants tested exhibited the transfer of all selected chromosomal markers at rather high (10(-4)-10(-6) per donor cell) but similar frequencies. Only one of the obtained donors, designated MFK 64, was… CONTINUE READING
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