Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.

@article{Matthysse1996ConstructionOG,
  title={Construction of GFP vectors for use in gram-negative bacteria other than Escherichia coli.},
  author={Ann G. Matthysse and Serina Stretton and Catherine E Dandie and Nicholas C. McClure and Amanda E. Goodman},
  journal={FEMS microbiology letters},
  year={1996},
  volume={145 1},
  pages={
          87-94
        }
}
A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519nfp, broad host range mob+ plasmids containing gfp expressed from a lac and an npt2 promoter, respectively. 

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