Construction of Escherichia coli K12 phr deletion and insertion mutants by gene replacement.

@article{Akasaka1991ConstructionOE,
  title={Construction of Escherichia coli K12 phr deletion and insertion mutants by gene replacement.},
  author={Susumu Akasaka and Kazuo Yamamoto},
  journal={Mutation research},
  year={1991},
  volume={254 1},
  pages={27-35}
}
We replaced an Escherichia coli phr gene by a 1.4-kb fragment of DNA coding for resistance to chloramphenicol. Characterization of 2 deletions (phr-19 and phr-36) and 1 insertion (phr-34) in the phr gene revealed no photoreactivation. Photoreactivation-deficient strains of either recA56 or lexA1(ind-) were more sensitive to UV radiation in the dark than phr-proficient counterparts. The presence of the phr defect in uvrA6 strains increased by 1.5-2-fold his-4(Ochre) to His+ mutation induced by… CONTINUE READING

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