Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

@article{Baba2006ConstructionOE,
  title={Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection},
  author={T. Baba and Takeshi Ara and M. Hasegawa and Yuki Takai and Yoshiko Okumura and Miki Baba and K. Datsenko and M. Tomita and B. Wanner and H. Mori},
  journal={Molecular Systems Biology},
  year={2006},
  volume={2},
  pages={2006.0008 - 2006.0008}
}
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. [...] Key Result Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of…Expand
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