Construction of Beauveria bassiana T-DNA insertion mutant collections and identification of thermosensitive and osmosensitive mutants.

Abstract

OBJECTIVE This study was to identify genes involved in thermotolerance and osmotolerance of Beauveria bassiana. METHODS A collection of T-DNA random insertion mutagenesis was constructed and thermosensitive and osmosensitive mutants were screened. The DNA fragments flanking to inserted T-DNA sequence in the mutants were isolated by using Y-shaped adaptor… (More)

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