Construction and use of recombinant Escherichia coli strains for the synthesis of toluene cis-glycol.

@article{Wahbi1996ConstructionAU,
  title={Construction and use of recombinant Escherichia coli strains for the synthesis of toluene cis-glycol.},
  author={L. P. Wahbi and David Gokhale and Steve Minter and Gill M. Stephens},
  journal={Enzyme and microbial technology},
  year={1996},
  volume={19 4},
  pages={297-306}
}
The toluene dioxygenase genes derived from Pseudomonas putida NCIMB 11767 were subcloned from a previously constructed recombinant plasmid, pIG, using pUC18 as the cloning vector and E. coli TG2 as the host strain. The resulting strain, E. coli TG2 (p1/1), produced toluene cis-glycol when grown in LB broth or minimal medium in the presence of toluene. Restriction mapping and partial DNA sequencing provided evidence for the presence of ORFs with extensive homology to parts of the tod operon from… CONTINUE READING