Construction and use of human chromosome jumping libraries from NotI-digested DNA

@article{Poustka1987ConstructionAU,
  title={Construction and use of human chromosome jumping libraries from NotI-digested DNA},
  author={Annemarie Poustka and T. Pohl and Denise P. Barlow and A. -M. Frischauf and Hans Lehrach},
  journal={Nature},
  year={1987},
  volume={325},
  pages={353-355}
}
A basic difficulty in the molecular analysis of genes identified by mutations in the mammalian genome is the need to cover genetic distances corresponding to several hundred kilobases or more by molecular techniques like chromosome walking. In chromosome jumping1–3, this limitation is overcome by the deletion of all but the extreme ends of large DNA molecules before cloning. We describe here the construction and characterization of a NotI 'jumping library' from human DNA. To characterize this… 

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