Construction and use of human chromosome jumping libraries from NotI-digested DNA

  title={Construction and use of human chromosome jumping libraries from NotI-digested DNA},
  author={Annemarie Poustka and T. Pohl and Denise P. Barlow and A. -M. Frischauf and Hans Lehrach},
A basic difficulty in the molecular analysis of genes identified by mutations in the mammalian genome is the need to cover genetic distances corresponding to several hundred kilobases or more by molecular techniques like chromosome walking. In chromosome jumping1–3, this limitation is overcome by the deletion of all but the extreme ends of large DNA molecules before cloning. We describe here the construction and characterization of a NotI 'jumping library' from human DNA. To characterize this… 

Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification

The dissection of the Langer-Giedion syndrome region on chromosome 8 from GTG-banded metaphase chromosomes and the universal enzymatic amplification of the dissected DNA is described, demonstrating that thousands of region-specific probes can be isolated within ten days.

Construction of a general human chromosome jumping library, with application to cystic fibrosis.

A general human chromosome jumping library was constructed that allows the cloning of DNA sequences approximately 100 kilobases away from any starting point in genomic DNA, and should now be applicable to any genetic locus for which a closely linked DNA marker is available.

Genomic DNA Libraries, Construction and Applications

By virtue of the powerful technology developed in molecular biology, it is possible to isolate any DNA fragment in the genome of an organism and, after reverse transcription, any transcribed gene in

Construction of Chromosome Jumping and Linking Libraries in E. coli

  • M. Drumm
  • Biology
    Current protocols in human genetics
  • 2001
Support protocols provide instructions for preparing genomic insert DNA, supF gene fragments, and Chromosome jumping allows the use of one point on a chromosome as a starting point for exploring another potentially distant point on the same chromosome.

Molecular analysis of the human MHC class I region using yeast artificial chromosome clones

End clones, which are of particular interest in the extension and refinement of the regional map, have been rescued by systematic subcloning of purified YACs and detailed rare-cutter restriction maps of the inserts have been generated.



Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.

  • F. CollinsS. Weissman
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1984
The principle of a DNA cloning procedure that directionally generates genomic DNA fragments 50-2000 kilobases away from an initial probe is presented and potential applications, such as mapping of complex genetic loci or moving from a linked gene toward a gene of interest, are presented and discussed.

A cell hybrid and recombinant DNA library that facilitate identification of polymorphic loci in the vicinity of the Huntington disease gene.

The cell hybrid and DNA library represent a rapid and efficient means to identify and isolate many polymorphic DNA markers close to and flanking the Huntington disease gene.

Long-range restriction site mapping of mammalian genomic DNA

Pulsed-field gradient gel electrophoresis is used to map sites for one class of infrequently cutting restriction enzyme over a total of 1,500 kb of mouse genomic DNA.

CpG-rich islands and the function of DNA methylation

It is likely that most vertebrate genes are associated with ‘HTF islands’—DNA sequences in which CpG is abundant and non-methylated. Highly tissue-specific genes, though, usually lack islands. The