Construction and usage of a onefold-coverage shotgun DNA microarray to characterize the metabolism of the archaeon Haloferax volcanii.

@article{Zaigler2003ConstructionAU,
  title={Construction and usage of a onefold-coverage shotgun DNA microarray to characterize the metabolism of the archaeon Haloferax volcanii.},
  author={Alexander Zaigler and Stephan C. Schuster and J{\"o}rg Soppa},
  journal={Molecular microbiology},
  year={2003},
  volume={48 4},
  pages={
          1089-105
        }
}
Haloferax volcanii is a moderately halophilic archaeon that can grow aerobically and anaerobically with a variety of substrates. We undertook a novel approach for the characterization of metabolic adaptations, i.e. transcriptome analysis with a onefold-coverage shotgun DNA microarray. A genomic library was constructed and converted into a polymerase chain reaction (PCR) product library, which was used to print two DNA microarrays, a 960-spot test array used for optimization of microarray… CONTINUE READING

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