Construction and screening of subtracted cDNA libraries from limited populations of plant cells: a comparative analysis of gene expression between maize egg cells and central cells.

@article{L2005ConstructionAS,
  title={Construction and screening of subtracted cDNA libraries from limited populations of plant cells: a comparative analysis of gene expression between maize egg cells and central cells.},
  author={Quy{\^e}n L{\^e} and Jose Gutierrez-Marcos and Liliana M. Costa and Stephanie D Meyer and H. G. Dickinson and Horst Loerz and Erhard Kranz and Stefan Scholten},
  journal={The Plant journal : for cell and molecular biology},
  year={2005},
  volume={44 1},
  pages={167-78}
}
The analysis of cell type-specific gene expression is an essential step in understanding certain biological processes during plant development, such as differentiation. Although methods for isolating specific cell types have been established, the application of cDNA subtraction to small populations of isolated cell types for direct identification of specific or differentially expressed transcripts has not yet been reported. As a first step in the identification of genes expressed differentially… CONTINUE READING

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