Construction and properties of Escherichia coli strains exhibiting -complementation of -galactosidase fragments in vivo.

@article{Zamenhof1972ConstructionAP,
  title={Construction and properties of Escherichia coli strains exhibiting -complementation of -galactosidase fragments in vivo.},
  author={P J Zamenhof and Merna Villarejo},
  journal={Journal of bacteriology},
  year={1972},
  volume={110 1},
  pages={171-8}
}
In vivo alpha-complementation of beta-galactosidase was demonstrated in 16 Z gene terminator (nonsense) mutant strains of Escherichia coli upon introduction of the episome F'M15 which specifies production of a mutant Z gene polypeptide containing a small deletion in the N-terminal region of the enzyme monomer. Genetic and biochemical analyses of the… CONTINUE READING