Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase Cζ

  title={Construction and primary analysis of human keratinocyte model expressing anti-sense protein kinase C$\zeta$},
  author={Xiaoyan Zhang and Huitu Liu and Shengqing Ma},
  journal={Chinese Science Bulletin},
Protein kinase C (PKC), which has a wide variety of functions, comprises at least twelve isoenzymes that have distinct roles respectively. Research in recent years indicates that PKCζ plays a pivotal role in the regulation of cell proliferation. In order to investigate its role in the regulation of keratinocyte proliferation, eukaryotic expression plasmid of anti-sense PKCζ is constructed and transfected into human keratinocyte line, Colo16. The human keratinocyte model expressing anti-sense… Expand
11 Citations
Characterization of free and bound bitumen fractions in a thermal maturation shale sequence. Part 1: Acidic and neutral compounds by negative-ion ESI FT-ICR MS
Abstract To evaluate petroleum generation and expulsion processes, pyrolysis experiments that simulate increased overburden pressures were conducted on an immature organic-rich shale with type-IIExpand
DRIFT spectroscopic study of diagenetic organic-clay interactions in argillaceous source rocks.
The correlation between the diagenetic smectite illitization and the decrease in the amount of combined OM leads to the conclusion that the smectitization may be a driving force for the OM desorption. Expand
Smectite-illitization difference of source rocks developed in saline and fresh water environments and its influence on hydrocarbon generation: A study from the Shahejie Formation, Dongying Depression, China
Abstract Samples of argillaceous source rocks from three sub-members of the Shahejie Formation (Es) in the Dongying Depression, China, were collected to investigate the differences in hydrocarbonExpand
Discussion on the method for determining BET specific surface area in argillaceous source rocks
Samples of argillaceous source rocks were taken at different depth in Dongying Sag and pretreated with different methods. Contrastive analysis to those samples by using N2 adsorption, pyrolysis,Expand
The relationship between organic matter and specific surface area in <2 μm clay size fraction of muddy source rock
The adsorption of organic matter (OM) onto clay minerals has long been considered as a significant way of OM preservation in source rock. Here we analyzed the relationship between OM and the specificExpand
A geochemical investigation of the free and carbonate-bound organic matter in the clay-sized fraction of argillaceous source rocks and its significance for biogenic interpretation
The molecular composition and biomarker distribution of various occurrences of organic matter in argillaceous source rocks developed in fresh and saline lacustrine environments were revealed byExpand
Movable oil content evaluation of lacustrine organic-rich shales: Methods and a novel quantitative evaluation model
Abstract Lacustrine shale oil resources are abundant and play important roles in maintaining a sustainable economy. The uncertainty in the movable oil content of organic-rich shales with strongExpand
Factors influencing the specific surface areas of argillaceous source rocks
This paper integrates methods of nitrogen adsorption (N2-BET) and ethylene glycol monoethyl ether (EGME) to present a comprehensive understanding of the specific surface areas (SSAs) of bulk rocksExpand
Prospect of shale gas recovery enhancement by oxidation-induced rock burst
Abstract By horizontal well multi-staged fracturing technology, shale rocks can be broken to form fracture networks via hydraulic force and increase the production rate of shale gas wells.Expand
Influence of organic matter on smectite illitization: A comparison between red and dark mudstones from the Dongying Depression, China
Abstract Interactions between organic matter (OM) and clay minerals have received considerable attention in previous studies. The inflence of OM on smectite illitization has been analyzed primarilyExpand


Intracellular targeting of protein kinase C isoenzymes: functional implications.
The activation of PKC 7 by cholesterol sulphate and PKC 5 by phosphatidylinositol trisphosphate in vitro suggests the possibility that activation of individual PKCs by different second messenger pathways in vivo may result in isoenzyme-specific responses. Expand
Protein kinase C--a question of specificity.
This review focuses on the heterogeneity within the PKC family and highlights some of the recent evidence that the isotypes might have separate and unique functions in the cell. Expand
The molecular heterogeneity of protein kinase C and its implications for cellular regulation
Protein kinase C is now known to be a large family of proteins, with multiple subspecies that have subtle individual enzymological characteristics, and probably have distinct functions in the processing and modulation of a variety of physiological and pathological responses to external signals. Expand
Cell cycle control in mammalian cells: role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs).
In this review, the mechanisms by which the mammalian cell cycle engine integrates intracellular and extracellular signals of different nature are discussed. Expand
Evidence for a role of MEK and MAPK during signal transduction by protein kinase C zeta.
It is shown here that MAPK and MEK are activated in vivo by an active mutant of zeta PKC, and that a kinase‐defective dominant negative mutant of zeppelin PKC dramatically impairs the activation of both MEK and MAPK by serum and tumour necrosis factor (TNF alpha). Expand
Protein kinase C: a family of isoenzymes with distinct roles in pathogenesis
Gene therapy is an unlikely treatment for diseases such as cancer, which have a significant non-genetic component2; the range of diseases that are realistically amenable is limited. Expand
Activation of protein kinase C by phosphatidylinositol 3,4,5-trisphosphate.
PIP3 by activating PKC may initiate a cascade of events from PIP3-->PKC- activation-->effects on other protein kinases such as MAP-kinase-->gene expression, and suggest a physiological role for PIP 3 in signal transduction. Expand
Diagnosis of genital infection caused by human papillomavirus using in situ hybridisation: the importance of the size of the biopsy specimen.
For a diagnosis of HPV infection using in situ hybridisation, the minimum length of epithelium in a cervical biopsy specimen should be 5 mm, and for high grade squamous intraepithelial lesions, specimens over 5 mm in length are suitable. Expand
Molecular Cloning: A Laboratory Manual
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Expand