Construction and performance of a rapid scan monochromator for multiwavelength fluorimetry.

@article{Hartmann1992ConstructionAP,
  title={Construction and performance of a rapid scan monochromator for multiwavelength fluorimetry.},
  author={Thomas Hartmann and Alan S. Verkman},
  journal={Analytical biochemistry},
  year={1992},
  volume={200 1},
  pages={139-42}
}
A rapid scan monochromator was constructed for multiwavelength illumination of biological samples. A light weight holographic grating (2400 grooves/mm) was driven by a rapid stepper motor with 0.014 degrees angular resolution in an f/4 monochromator. Angular velocity and acceleration kinetics were optimized to minimize the time for grating rotation and… CONTINUE READING