Construction and organization of a BSL-3 cryo-electron microscopy laboratory at UTMB.

  title={Construction and organization of a BSL-3 cryo-electron microscopy laboratory at UTMB.},
  author={M. Sherman and J. Trujillo and Ian A. Leahy and Dennis Razmus and Robert Dehate and Paul Lorcheim and Mark A. Czarneski and Domenica Zimmerman and JeT'Aime M Newton and A. Haddow and S. Weaver},
  journal={Journal of structural biology},
  volume={181 3},
A unique cryo-electron microscopy facility has been designed and constructed at the University of Texas Medical Branch (UTMB) to study the three-dimensional organization of viruses and bacteria classified as select agents at biological safety level (BSL)-3, and their interactions with host cells. A 200keV high-end cryo-electron microscope was installed inside a BSL-3 containment laboratory and standard operating procedures were developed and implemented to ensure its safe and efficient… Expand
8 Citations
Cryofixation of Inactivated Hantavirus-Infected Cells as a Method for Obtaining High-Quality Ultrastructural Preservation for Electron Microscopic Studies
Structures of enveloped virions determined by cryogenic electron microscopy and tomography
  • 4
Laboratory Safety, Biosecurity, and Responsible Animal Use.
  • 1
  • PDF


A Unique BSL-3 Cryo-Electron Microscopy Laboratory at UTMB
  • 4
  • PDF
Cryoprotection in electron microscopy
  • 1986
  • 27
Cryo-electron microscopy of vitrified specimens.
  • 1,859
  • PDF
Adding the Third Dimension to Virus Life Cycles: Three-Dimensional Reconstruction of Icosahedral Viruses from Cryo-Electron Micrographs
  • 524
  • PDF
Cryo-electron microscopy reveals the functional organization of an enveloped virus, Semliki Forest virus.
  • 209
Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy.
  • 1,445
  • Highly Influential
  • PDF
Placement of the Structural Proteins in Sindbis Virus
  • 206
  • Highly Influential
  • PDF
Three-Dimensional Organization of Rift Valley Fever Virus Revealed by Cryoelectron Tomography
  • 105
  • PDF
EMAN2: an extensible image processing suite for electron microscopy.
  • 2,293
  • PDF