Construction and in vivo analysis of new split lactose permeases.

  title={Construction and in vivo analysis of new split lactose permeases.},
  author={W Wrubel and U Stochaj and Ruth Ehring},
  journal={FEBS letters},
  volume={349 3},
The capacity of incomplete segments of Escherichia coli lactose permease to form transport-competent complexes in vivo was further tested. Two series of mutant lacY genes were constructed. One encoded N-terminal lactose permease segments of different length. The proteins specified by the other group contained deletions of different length and location within the N-terminal region. Several pairs of such mutant proteins reconstituted active lactose transport. For certain combinations duplications… CONTINUE READING

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