Construction and expression of recombinant plasmid pCD-rbFGF in osteoblasts

  title={Construction and expression of recombinant plasmid pCD-rbFGF in osteoblasts},
  author={Yuanpeng Cao and Yang Shuhua and Guo Xiaodong and Qu Shen},
  journal={Journal of Huazhong University of Science and Technology [Medical Sciences]},
To construct basic fibroblast growth factor (bFGF) eukaryotic expression vector and to evaluate the possibility of bFGF gene therapy in orthopedic disease, the pCD-rbFGF recombinant plasmid was constructed by cloning rat basic fibroblast growth factor (bFGF) cDNA into an eukaryotic expression vector, pcDNA3. Rat osteoblasts were transfected with pCD-rbFGF plasmid by lopofectin mediated gene transfer, the transient expression was detected by streptavidin-biotin-enzyme complex (SABC) method. It… CONTINUE READING