Construction and detection of expression vectors of microRNA-9a in BmN cells

@article{Huang2011ConstructionAD,
  title={Construction and detection of expression vectors of microRNA-9a in BmN cells},
  author={Yong Can Huang and Quan Zou and Sheng-peng Wang and Shun-ming Tang and Guo-zheng Zhang and Xing-jia Shen},
  journal={Journal of Zhejiang University SCIENCE B},
  year={2011},
  volume={12},
  pages={527-533}
}
MicroRNAs (miRNAs) are small endogenous RNAs molecules, approximately 21–23 nucleotides in length, which regulate gene expression by base-pairing with 3′ untranslated regions (UTRs) of target mRNAs. However, the functions of only a few miRNAs in organisms are known. Recently, the expression vector of artificial miRNA has become a promising tool for gene function studies. Here, a method for easy and rapid construction of eukaryotic miRNA expression vector was described. The cytoplasmic actin 3… CONTINUE READING
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