• Corpus ID: 38229427

Construction and characterization of genomic libraries from specific human chromosomes ( human chromosomes 21 and 22 / gene mapping / chromosome sorting / recombinant DNA / flow cytometry )

@inproceedings{Krumlauf2003ConstructionAC,
  title={Construction and characterization of genomic libraries from specific human chromosomes ( human chromosomes 21 and 22 / gene mapping / chromosome sorting / recombinant DNA / flow cytometry )},
  author={R. Krumlauf and Marc JEANPIERREt and Bryan D. Young},
  year={2003}
}
Highly purified fractions ofhuman chromosomes 21 and 22 were isolated from a suspension of metaphase chromosomes stained with ethidium bromide by using a fluorescenceactivated cell sorter (FACS II). Two recombinant DNA libraries, representing chromosomes 21 and 22, were constructed-by complete digestion of DNA from these fractions with EcoRI and insertion into the vector AgtWESAB. Twenty clones selected at random from the chromosome 22 library hybridized to EcoRIdigested human DNA, and five… 

Figures from this paper