Construction and characterization of an acapsular mutant of Mannheimia haemolytica A1.

@article{McKerral2002ConstructionAC,
  title={Construction and characterization of an acapsular mutant of Mannheimia haemolytica A1.},
  author={Linda J. McKerral and Reggie Y. C. Lo},
  journal={Infection and immunity},
  year={2002},
  volume={70 5},
  pages={2622-9}
}
The nmaA and nmaB genes, which code for UDP-GlcNAc-2-epimerase and UDP-ManNAc-dehydrogenase, respectively, are involved in capsular polysaccharide biosynthesis in Mannheimia haemolytica A1. A chloramphenicol resistance (Cm(r)) cassette cloned behind an M. haemolytica A1 promoter, plpcat, was created and used to interrupt nmaA and nmaB. A 1.3-kbp DNA fragment that encompasses part of nmaA and nmaB was replaced by the 1.0-kbp plpcat, resulting in a knockout mutant which is Cm(r) and unable to… CONTINUE READING

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