Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

@article{Chang1978ConstructionAC,
  title={Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.},
  author={Annie C. Y. Chang and Stanley N. Cohen},
  journal={Journal of bacteriology},
  year={1978},
  volume={134 3},
  pages={1141-56}
}
Construction and characterization of a class of multicopy plasmid cloning vehicles containing the replication system of miniplasmid P15A are described. The constructed plasmids have cleavage sites within antibiotic resistance genes for a variety of commonly employed site-specific endonucleases, permitting convenient use of the insertional inactivation procedure for the selection of clones that contain hybrid DNA molecules. Although the constructed plasmids showed DNA sequence homology with the… CONTINUE READING
Highly Influential
This paper has highly influenced 56 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Related Discussions
This paper has been referenced on Twitter 2 times. VIEW TWEETS

Citations

Publications citing this paper.
Showing 1-10 of 1,023 extracted citations

A simple and rapid method of bacterial transformation.

Journal of microbiological methods • 2010
View 4 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…