Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.

@article{Salmons1989ConstructionAC,
  title={Construction and characterization of a packaging cell line for MMTV-based conditional retroviral vectors.},
  author={Brian Salmons and S Moritz-Legrand and I Garcha and Walter H. G{\"u}nzburg},
  journal={Biochemical and biophysical research communications},
  year={1989},
  volume={159 3},
  pages={1191-8}
}
A chimeric provirus in which the 5'LTR of a complete biologically active Mouse Mammary Tumour Virus (MMTV) proviral DNA has been replaced with the Rous Sarcoma Virus LTR has been constructed. Upon transfection into permissive cells, this provirus directs the synthesis of the MMTV gag and env structural proteins, but is impaired in packaging of the RNAs that encode these proteins. Supertransfection of these cells with MMTV based vector constructs results in the production of infectious… CONTINUE READING
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