Construction and characterization of a highly complex retroviral library for lineage analysis.

@article{Golden1995ConstructionAC,
  title={Construction and characterization of a highly complex retroviral library for lineage analysis.},
  author={Jeffrey Alan Golden and S C Fields-Berry and Constance L. Cepko},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 12},
  pages={5704-8}
}
Replication-incompetent retroviral vectors encoding histochemical reporter genes have been used for studying lineal relationships in a variety of species. A crucial element in the interpretation of data generated by this method is the identification of sibling relationships, or clonal boundaries. The use of a library of viruses in which each member is unique can greatly facilitate this aspect of the analysis. A previously reported murine retroviral library containing about 80 members… CONTINUE READING