Construction and characterization of a full-lengh cDNA library from non-fresh Giardia lamblia.

@article{Guo2012ConstructionAC,
  title={Construction and characterization of a full-lengh cDNA library from non-fresh Giardia lamblia.},
  author={Jun-li Guo and Jie Jiang and Wen-yu Zheng and Ming-Luan Li and Xi-feng Tian and Xian-min Feng and Yue-hua Wang and Xiao-hong Ju and Yue-Qiong Kong},
  journal={Asian Pacific journal of tropical medicine},
  year={2012},
  volume={5 12},
  pages={931-4}
}
OBJECTIVE To construct rapidly a full-length cDNA library from nanogram amounts total RNA of Giardia lamblia (G. lamblia) trophozoites stocked in RNA stabilization reagent. METHODS Total RNA of Giardia was extracted using Trizol reagent. A full-length cDNA library of G. lamblia trophozoites was constructed by a long-distance PCR (LD-PCR) method. The recombinant rate and the coverage rate of full-length clones of the library were evaluated. The inserted fragments were identified and sequenced… CONTINUE READING

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