Construction and characterization of a derivative of Neisseria gonorrhoeae strain MS11 devoid of all opa genes.

@article{LeVan2012ConstructionAC,
  title={Construction and characterization of a derivative of Neisseria gonorrhoeae strain MS11 devoid of all opa genes.},
  author={Adriana LeVan and Lindsey I Zimmerman and Amanda Caroline Mahle and Karen V Swanson and Philip DeShong and Juhee Park and Vonetta L Edwards and Wenxia Song and D. C. Stein},
  journal={Journal of bacteriology},
  year={2012},
  volume={194 23},
  pages={
          6468-78
        }
}
To better understand the role of Opa in gonococcal infections, we created and characterized a derivative of MS11 (MS11Δopa) that had the coding sequence for all 11 Opa proteins deleted. The MS11Δopa bacterium lost the ability to bind to purified lipooligosaccharide (LOS). While nonpiliated MS11Δopa and nonpiliated Opa-expressing MS11 cells grew at the same rate, nonpiliated MS11Δopa cells rarely formed clumps of more than four bacteria when grown in broth with vigorous shaking. Using flow… CONTINUE READING

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Rapid transfer of DNA from agarose gels to nylon membranes.

  • Nucleic acids research
  • 1985
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