Construction and characterization of a bovine BAC library with four genome-equivalent coverage

@article{Eggen2001ConstructionAC,
  title={Construction and characterization of a bovine BAC library with four genome-equivalent coverage},
  author={Andr{\'e} Eggen and Mathieu Gautier and Alain Billaut and Elisabeth J. Petit and H{\'e}l{\`e}ne Hayes and P. J. Laurent and Catherine Urban and Martha Pfister-Genskow and Ken Eilertsen and Michael D. Bishop},
  journal={Genetics, Selection, Evolution : GSE},
  year={2001},
  volume={33},
  pages={543 - 548}
}
A bovine artificial chromosome (BAC) library of 105 984 clones has been constructed in the vector pBeloBAC11 and organized in 3-dimension pools and high density membranes for screening by PCR and hybridization. The average insert size, determined after analysis of 388 clones, was estimated at 120 kb corresponding to a four genome coverage. Given the fact that a male was used to construct the library, the probability of finding any given autosomal and X or Y locus is respectively 0.98 and 0.86… CONTINUE READING