Construction and characterization of a DNA probe for distinguishing strains of Aspergillus flavus.

@article{McAlpin1995ConstructionAC,
  title={Construction and characterization of a DNA probe for distinguishing strains of Aspergillus flavus.},
  author={Cesaria E. McAlpin and Bruno M. Mannarelli},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 3},
  pages={1068-72}
}
Repetitive DNA sequences have proven useful and reliable characters in evaluating genetic relatedness of strains at different levels of taxonomic classification. A DNA probe was constructed to distinguish among strains of Aspergillus flavus by DNA fingerprinting techniques. Chromosomal DNA of A. flavus var. flavus NRRL 6541 was partially digested with EcoRI and ligated to a Lambda Dash bacteriophage vector. Four lambda clones were identified which displayed multiple and distinct bands when… CONTINUE READING

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