Construction and applications of yellow fever virus replicons.

  title={Construction and applications of yellow fever virus replicons.},
  author={Christopher Tyler Jones and Chinmay G Patkar and Richard J. Kuhn},
  volume={331 2},
Subgenomic replicons of yellow fever virus (YFV) were constructed to allow expression of heterologous reporter genes in a replication-dependent manner. Expression of the antibiotic resistance gene neomycin phosphotransferase II (Neo) from one of these YFV replicons allowed selection of a stable population of cells (BHK-REP cells) in which the YFV replicon persistently replicated. BHK-REP cells were successfully used to trans-complement replication-defective YFV replicons harboring large… CONTINUE READING