Construction and applications of a highly transmissible murine retrovirus shuttle vector

@article{Cepko1984ConstructionAA,
  title={Construction and applications of a highly transmissible murine retrovirus shuttle vector},
  author={Constance L. Cepko and B. E. Roberts and Richard C. Mulligan},
  journal={Cell},
  year={1984},
  volume={37},
  pages={1053-1062}
}

Retrovirus Vectors and Regulatable Promoters

Transfer of therapeutic genes into diseased cells using retroviral vectors using Moloney murine leukemia virus is a promising approach for the treatment of certain, virtually incurable diseases.

Construction and characterization of a retroviral vector demonstrating efficient expression of cloned cDNA sequences.

The pMV-7 vector is capable of high-efficiency transfer and high-frequency expression of the cDNA-encoded protein and contains the selectable drug resistance gene neo under the regulation of the herpes simplex virus (HSV) thymidine kinase (tk) promoter.

Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells.

A retrovirus-derived vector called self-inactivating (SIN) vector was designed for the transduction of whole genes into mammalian cells and led to the formation of an authentic mRNA transcript, which in some cases was induced by cadmium.

Methods for the construction of retroviral vectors and the generation of high-titer producers.

Retroviral vectors are powerful tools for gene transfer that are useful in the context of experimental as well as clinical applications, and murine leukemia virus-based vectors accommodate numerous modifications, thus providing a plastic tool that can be tailored for very diverse applications.

Characterization of a retrovirus shuttle vector capable of either proviral integration or extrachromosomal replication in mouse cells

The data presented here suggest that these retroviruses should be useful as gene transfer vectors for animal cells in culture or in vivo.
...

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