Construction and applications of a highly transmissible murine retrovirus shuttle vector
@article{Cepko1984ConstructionAA, title={Construction and applications of a highly transmissible murine retrovirus shuttle vector}, author={Constance L. Cepko and B. E. Roberts and Richard C. Mulligan}, journal={Cell}, year={1984}, volume={37}, pages={1053-1062} }
848 Citations
Retrovirus Vectors and Regulatable Promoters
- Biology
- 1998
Transfer of therapeutic genes into diseased cells using retroviral vectors using Moloney murine leukemia virus is a promising approach for the treatment of certain, virtually incurable diseases.
Construction and characterization of a retroviral vector demonstrating efficient expression of cloned cDNA sequences.
- BiologyDNA
- 1988
The pMV-7 vector is capable of high-efficiency transfer and high-frequency expression of the cDNA-encoded protein and contains the selectable drug resistance gene neo under the regulation of the herpes simplex virus (HSV) thymidine kinase (tk) promoter.
Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1986
A retrovirus-derived vector called self-inactivating (SIN) vector was designed for the transduction of whole genes into mammalian cells and led to the formation of an authentic mRNA transcript, which in some cases was induced by cadmium.
Shuttle vector system for the analysis of mutational events in mammalian chromosomal DNA.
- BiologyMutation research
- 1989
Introduction of sequences encoding functional human adenosine deaminase into mouse cells using a retroviral shuttle system.
- Biology, EngineeringGene
- 1985
Methods for the construction of retroviral vectors and the generation of high-titer producers.
- Biology, EngineeringMethods in molecular medicine
- 1997
Retroviral vectors are powerful tools for gene transfer that are useful in the context of experimental as well as clinical applications, and murine leukemia virus-based vectors accommodate numerous modifications, thus providing a plastic tool that can be tailored for very diverse applications.
Characterization of a retrovirus shuttle vector capable of either proviral integration or extrachromosomal replication in mouse cells
- BiologyMolecular and cellular biology
- 1985
The data presented here suggest that these retroviruses should be useful as gene transfer vectors for animal cells in culture or in vivo.
References
SHOWING 1-10 OF 54 REFERENCES
Construction of Highly Transmissible Mammalian Cloning Vehicles Derived from Murine Retroviruses
- Biology
- 1983
Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene
- BiologyMolecular and cellular biology
- 1982
Great general applicability of retroviruses as eucaryotic vectors is suggested, as the chimeric MLV-tk virus showed single-hit kinetics in infections and behaved like known replication-defective retrovirus.
Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus
- BiologyCell
- 1983
Splicing of intervening sequences introduced into an infectious retroviral vector.
- BiologyJournal of molecular and applied genetics
- 1982
The v-src gene was removed from Rous sarcoma virus DNA and replaced with either a cDNA or genomic clone of human alpha-chorionic gonadotropin, creating recombinants carrying a perfect cDNA copy of the original genomic insert.
Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells.
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 1982
The expression of BPV-linked cellular genes in conjunction with the ability to shuttle DNA between bacteria and mammalian cells may provide a rapid means of analyzing and recovering genes that confer an identifiable phenotype upon mammalian cells.
Retrovirus transduction: generation of infectious retroviruses expressing dominant and selectable genes is associated with in vivo recombination and deletion events
- BiologyMolecular and cellular biology
- 1983
These results demonstrate that nonhomologous recombination and deletion events can take place in animal cells, resulting in the acquisition or removal of cis-acting sequences required for, or inhibitory to, retrovirus infectivity.
Retroviruses as mutagens: Insertion and excision of a nontransforming provirus alter expression of a resident transforming provirus
- BiologyCell
- 1981
Identification of DNA sequences required for transcription of the human α1-globin gene in a new SV40 host-vector system
- BiologyCell
- 1981
Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1
- BiologyJournal of virology
- 1981
It is concluded that retroviruses can be used as true vectors for genes other than genes that lead to oncogenesis and could be rescued into virus particles at high titers by superinfection with a helper-independent retrovirus.