Construction and applications of a highly transmissible murine retrovirus shuttle vector

  title={Construction and applications of a highly transmissible murine retrovirus shuttle vector},
  author={Constance L. Cepko and B. E. Roberts and Richard C. Mulligan},

Retrovirus Vectors and Regulatable Promoters

Transfer of therapeutic genes into diseased cells using retroviral vectors using Moloney murine leukemia virus is a promising approach for the treatment of certain, virtually incurable diseases.

Construction and characterization of a retroviral vector demonstrating efficient expression of cloned cDNA sequences.

The pMV-7 vector is capable of high-efficiency transfer and high-frequency expression of the cDNA-encoded protein and contains the selectable drug resistance gene neo under the regulation of the herpes simplex virus (HSV) thymidine kinase (tk) promoter.

Self-inactivating retroviral vectors designed for transfer of whole genes into mammalian cells.

A retrovirus-derived vector called self-inactivating (SIN) vector was designed for the transduction of whole genes into mammalian cells and led to the formation of an authentic mRNA transcript, which in some cases was induced by cadmium.

Methods for the construction of retroviral vectors and the generation of high-titer producers.

Retroviral vectors are powerful tools for gene transfer that are useful in the context of experimental as well as clinical applications, and murine leukemia virus-based vectors accommodate numerous modifications, thus providing a plastic tool that can be tailored for very diverse applications.

Characterization of a retrovirus shuttle vector capable of either proviral integration or extrachromosomal replication in mouse cells

The data presented here suggest that these retroviruses should be useful as gene transfer vectors for animal cells in culture or in vivo.



Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene

Great general applicability of retroviruses as eucaryotic vectors is suggested, as the chimeric MLV-tk virus showed single-hit kinetics in infections and behaved like known replication-defective retrovirus.

Splicing of intervening sequences introduced into an infectious retroviral vector.

The v-src gene was removed from Rous sarcoma virus DNA and replaced with either a cDNA or genomic clone of human alpha-chorionic gonadotropin, creating recombinants carrying a perfect cDNA copy of the original genomic insert.

Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells.

The expression of BPV-linked cellular genes in conjunction with the ability to shuttle DNA between bacteria and mammalian cells may provide a rapid means of analyzing and recovering genes that confer an identifiable phenotype upon mammalian cells.

Retrovirus transduction: generation of infectious retroviruses expressing dominant and selectable genes is associated with in vivo recombination and deletion events

These results demonstrate that nonhomologous recombination and deletion events can take place in animal cells, resulting in the acquisition or removal of cis-acting sequences required for, or inhibitory to, retrovirus infectivity.

Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1

It is concluded that retroviruses can be used as true vectors for genes other than genes that lead to oncogenesis and could be rescued into virus particles at high titers by superinfection with a helper-independent retrovirus.