Construction and applications of a highly transmissible murine retrovirus shuttle vector

@article{Cepko1984ConstructionAA,
  title={Construction and applications of a highly transmissible murine retrovirus shuttle vector},
  author={C. Cepko and B. Roberts and R. Mulligan},
  journal={Cell},
  year={1984},
  volume={37},
  pages={1053-1062}
}
We develop a murine retrovirus shuttle vector system for the efficient introduction of selectable and nonselectable DNA sequences into mammalian cells and recovery of the inserted sequences as molecular clones. [...] Key Result Three protocols allow rapid recovery of vector DNA sequences from mammalian cells. Two of the methods rely on SV40 T-antigen-mediated replication of the vector sequences and yield thousands of bacterial transformants per 5 X 10(6) mammalian cells.Expand
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  • S. Yu, T. von Rüden, +6 authors E. Gilboa
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1986
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TLDR
The properties of the shuttle vector system were particularly useful for analysis of the molecular mechanisms of mutational events in chromosomal DNA of mammalian cells. Expand
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Genetics and Biology of Retroviral Vectors
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TLDR
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Characterization of a retrovirus shuttle vector capable of either proviral integration or extrachromosomal replication in mouse cells
A retrovirus shuttle vector is described that contains the dominant selectable neo gene which confers resistance to kanamycin in bacteria and to the drug G418 in animal cells. The bacterial supF geneExpand
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The retrovirus vector system described appears to possess a number of distinct advantages over currently available methods for introducing DNA sequences into mammalian cells, including the ability to represent a large number of independent cloned DNA molecules as infectious viruses. Expand
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