Construction and applications of a highly transmissible murine retrovirus shuttle vector

@article{Cepko1984ConstructionAA,
  title={Construction and applications of a highly transmissible murine retrovirus shuttle vector},
  author={C. Cepko and B. Roberts and R. Mulligan},
  journal={Cell},
  year={1984},
  volume={37},
  pages={1053-1062}
}
We develop a murine retrovirus shuttle vector system for the efficient introduction of selectable and nonselectable DNA sequences into mammalian cells and recovery of the inserted sequences as molecular clones. [...] Key Result Three protocols allow rapid recovery of vector DNA sequences from mammalian cells. Two of the methods rely on SV40 T-antigen-mediated replication of the vector sequences and yield thousands of bacterial transformants per 5 X 10(6) mammalian cells.Expand
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