Construction and application of a yeast expression system for thymosin alpha1.

@article{Chen2005ConstructionAA,
  title={Construction and application of a yeast expression system for thymosin alpha1.},
  author={Feng Chen and Xiang-ming Chen and Zhi Chen and Han-liang Jiang and Xiao-Ping Pan and Zhong-rong Hu and Rong-hua Liu and Xiao-Ming Chen},
  journal={Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al},
  year={2005},
  volume={29 3},
  pages={253-9}
}
We want to construct a yeast expression system for thymosin alpha1 (Talpha1) to make the orally administered Talpha1 preparation possible. The whole Talpha1 DNA fragment was obtained by PCR. After being digested with restriction enzymes, it was cloned into pYES2 vector. Sequencing was performed to identify the recombinant. The sequence of Talpha1 in recombinant coincided with the original one reported in Genbank. When pYES2-Talpha1 plasmid was transformed into yeast, galactose instead of… CONTINUE READING