[Construction and analysis of the tularemia pathogen gene library in Escherichia coli].

Abstract

The library of tularemia causative agent genes cloned on the pHC79 plasmid and the partial clonotek of these agents genes in Escherichia coli cells have been constructed. The immunochemical analysis has revealed seven clones of Escherichia coli harbouring the recombinant plasmids and expressing francisella antigens. The cloned sequences of francisella DNA as well as the recombinant plasmids containing them and coding for francisella antigens are capable of specific hybridization with the DNA from Francisella tularensis strains and Francisella novicida strain U112. The cloned DNA sequences have the properties of the genetic radiospecific molecular DNA probe.

Cite this paper

@article{Zakharenko1990ConstructionAA, title={[Construction and analysis of the tularemia pathogen gene library in Escherichia coli].}, author={V. I. Zakharenko and A. V. Nenashev and M S Govorun and Ludmila V. Komissarova and G. I. Aleshkin and A G Skavronskaia}, journal={Molekuliarnaia genetika, mikrobiologiia i virusologiia}, year={1990}, volume={2}, pages={22-5} }