Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage.

  title={Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage.},
  author={Oded B{\'e}j{\`a} and M. T. Suzuki and Eugene V. Koonin and L. Aravind and Andrew G. Hadd and L P Nguyen and Roosevelt Garc{\'i}a Villacorta and Motahareh Ala Amjadi and Christel Garrigues and Stevan B. Jovanovich and Robert Alan Feldman and Edward F. Delong},
  journal={Environmental microbiology},
  volume={2 5},
Cultivation-independent surveys of ribosomal RNA genes have revealed the existence of novel microbial lineages, many with no known cultivated representatives. Ribosomal RNA-based analyses, however, often do not provide significant information beyond phylogenetic affiliation. Analysis of large genome fragments recovered directly from microbial communities represents one promising approach for characterizing uncultivated microbial species better. To assess further the utility of this approach, we… 

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