Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus.

@article{Takahama2003ConstructionAA,
  title={Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus.},
  author={Kazutaka Takahama and Masayoshi Matsuoka and Kazuhiro Nagahama and Takahira Ogawa},
  journal={Journal of bioscience and bioengineering},
  year={2003},
  volume={95 3},
  pages={302-5}
}
The coding sequence of a gene for a Pseudomonas syringae ethylene-forming enzyme was inserted at the psbAI locus in a cyanobacterium, Synechococcus elongatus PCC 7942 via rps12-mediated gene replacement. The recombinant strain photoautotrophically produced ethylene at 451 nl ml(-1) h(-1) OD730(-1), but showed a depressed specific growth rate as well as a… CONTINUE READING