Construction and Preliminary Analysis of a Deep-Sea Sediment Metagenomic Fosmid Library from Qiongdongnan Basin, South China Sea

  title={Construction and Preliminary Analysis of a Deep-Sea Sediment Metagenomic Fosmid Library from Qiongdongnan Basin, South China Sea},
  author={Yongfei Hu and Chengzhang Fu and Yeshi Yin and Gong Cheng and Fang Lei and Xi Yang and Jing Li and Elizabeth Jane Ashforth and Lixin Zhang and Baoli Zhu},
  journal={Marine Biotechnology},
Preliminary characterization of the microbial phylogeny and metabolic potential of a deep-sea sediment sample from the Qiongdongnan Basin, South China Sea, was carried out using a metagenomic library approach. An effective and rapid method of DNA isolation, purification, and library construction was used resulting in approximately 200,000 clones with an average insert size of about 36 kb. End sequencing of 600 individual clones from the fosmid library generated 1,051 sequences with an average… CONTINUE READING
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