Construction and Characterization of a Fluorescent Sendai Virus Carrying the Gene for Envelope Fusion Protein Fused with Enhanced Green Fluorescent Protein

@article{Miyazaki2010ConstructionAC,
  title={Construction and Characterization of a Fluorescent Sendai Virus Carrying the Gene for Envelope Fusion Protein Fused with Enhanced Green Fluorescent Protein},
  author={Masao Miyazaki and Hiroaki Segawa and Tetsuro Yamashita and Yafeng Zhu and Kaoru Takizawa and Mamoru Tsukuba-shi Hasegawa and Hideharu Taira},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2010},
  volume={74},
  pages={2293 - 2298}
}
Sendai virus (SeV) is an enveloped virus with a non-segmented negative-strand RNA genome. SeV envelope fusion (F) glycoproteins play crucial roles in the viral life cycle in processes such as viral binding, assembly, and budding. In this study, we developed a viable recombinant SeV designated F-EGFP SeV/ΔF, in which the F protein was replaced by an F protein fused to EGFP at the carboxyl terminus. Living infected cells of the recombinant virus were directly visualized by green fluorescence. The… 
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