• Biology
  • Published 2007

Construction and Characterization of Recombinant Prokaryotic Vector pGEX-4T-1/γ-ggt

@inproceedings{Junfeng2007ConstructionAC,
  title={Construction and Characterization of Recombinant Prokaryotic Vector pGEX-4T-1/γ-ggt},
  author={Xu Jun-feng},
  year={2007}
}
To design and construct an prokaryotic expression vector pGEX-4T-1/γ-ggt,and to express Escherichia coli γ-glutamyl transpeptidase,the BglⅡand XhoⅠsites were incorporated into the γ-ggt encoding fragment by PCR.After digesting with BglⅡand XhoⅠ,the γ-ggt encoding fragment was cloned into the prokaryotic expression vector pGEX-4T-1 at the corresponding BamHⅠand XhoⅠsites.The positive clones selected with PCR were sequenced and the expression of γ-ggt in E.coli BL21(DE3) was analyzed with SDS… CONTINUE READING