Construction and Characterization of Escherichia coli Whole-Cell Biosensors for Toluene and Related Compounds

@article{Behzadian2010ConstructionAC,
  title={Construction and Characterization of Escherichia coli Whole-Cell Biosensors for Toluene and Related Compounds},
  author={Farida Behzadian and H. Barjeste and Saman Hosseinkhani and Ali Reza Zarei},
  journal={Current Microbiology},
  year={2010},
  volume={62},
  pages={690-696}
}
The XylR regulatory protein is a transcriptional activator from the TOL plasmid of Pseudomonas putida mt-2 that is involved in the toluene and benzene degradation pathway. Here we describe the construction and laboratory characterization of recombinant biosensors (pGLPX plasmids) based on XylR and its cognate promoter (Pu). In the pGLPX plasmid, the reporter luc gene is under the control of the Pu promoter. We evaluated the ability of two distinct nucleotide sequences to function as SD elements… CONTINUE READING

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