Construction, purification, and properties of a truncated alkaline endoglucanase from Bacillus sp. KSM-635.

@article{Ozaki1995ConstructionPA,
  title={Construction, purification, and properties of a truncated alkaline endoglucanase from Bacillus sp. KSM-635.},
  author={Katsuya Ozaki and Yutaro Hayashi and Nobuyuki Sumitomo and Shuji Kawai and Susumu Ito},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1995},
  volume={59 9},
  pages={1613-8}
}
Part of a 2.4-kb DNA fragment that encoded the amino-terminal 584 residues (65 kDa) of an alkaline endoglucanase from Bacillus sp. KSM-635 (941 amino acid residues; 105 kDa) was spontaneously deleted during subcloning of the fragment. The remaining 1.1-kb insert of the deleted plasmid encoded amino acids from Ala228 to Leu584 of the enzyme. However, Escherichia coli HB101 cells harboring this plasmid produced an active endoglucanase. After addition of a termination codon, TAA, immediately… CONTINUE READING